无纺网与多孔海绵状材料作为软骨组织工程支架的适用性及体内降解性 本文关键词：海绵状，多孔，软骨，降解，支架
无纺网与多孔海绵状材料作为软骨组织工程支架的适用性及体内降解性 本文简介：无纺网与多孔海绵状材料作为软骨组织工程支架的适用性及体内降解性孙安科1，孙靖2，孙伟1，张贺3，陈伟4，华泽权5解放军沈阳军区总医院，1耳鼻咽喉科，3实验动物中心，4医学实验科，5颌面外科，辽宁省沈阳市110016；2辽宁医学院2012级硕士研究生，辽宁省锦州市121001Applicability
无纺网与多孔海绵状材料作为软骨组织工程支架的适用性及体内降解性 本文内容：
无纺网与多孔海绵状材料作为软骨组织工程支架的适用性及体内降解性
孙安科1，孙
靖2，孙
伟1，张
贺3，陈
伟4，华泽权5
解放军沈阳军区总医院，1耳鼻咽喉科，3实验动物中心，4医学实验科，5颌面外科，辽宁省沈阳市
110016；2辽宁医学院2012级硕士研究生，辽宁省锦州市
121001
Applicability
and
biodegradability
in
vivo
upon
polyglycolic
acid
nonwoven
mesh
and
poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)
porous
sponge
as
scaffolds
for
tissue
engineered
cartilage
Sun
An-ke1,Sun
Jing2,Sun
Wei1,Zhang
He3,Chen
Wei4,Hua
Ze-quan5
1Department
of
Otorhinolaryngology,3Centre
for
Experimental
Animals,4Department
of
Medical
Laboratory,5Department
of
Maxillofacial
Surgery,General
Hospital
of
Shenyang
Military
Region,Shenyang
110016,Liaoning
Province,China;
2Grade
2012,Postgraduate
School,Liaoning
Medical
University,Jinzhou
121001,Liaoning
Province,China
摘要
背景：聚羟基乙酸无纺网与聚羟基丁酸酯-聚羟基己酸酯共聚物多孔海绵具有良好的塑形适应性、生物降解性与生物相容性。
目的：观察聚羟基乙酸无纺网与聚羟基丁酸酯-聚羟基己酸酯共聚物多孔海绵作为软骨组织工程支架的适用性及体内降解性。
方法：分别制备乳兔软骨细胞-聚羟基乙酸无纺网复合物、乳兔软骨细胞-聚羟基丁酸酯-聚羟基己酸酯共聚物多孔海绵复合物。在实验组成年兔两侧背部皮下分别植入制备的两种复合物，在对照组成年兔两侧背部皮下分别植入聚羟基乙酸无纺网与聚羟基丁酸酯-聚羟基己酸酯共聚物。
结果与结论：组织学观察显示，以聚羟基乙酸无纺网获取的组织工程软骨，植入4
周时软骨细胞较小，软骨内有较多聚羟基乙酸纤维残留，8周时软骨细胞较成熟，包埋在陷窝内，聚羟基乙酸纤维消失，12周时软骨细胞成熟，基质分泌丰富，无聚羟基乙酸存留；以聚羟基丁酸酯-聚羟基己酸酯共聚物多孔海绵获取的组织工程软骨，植入4周时软骨细胞不成熟，软骨基质内似“杂质”样材料残留物较多，8周时软骨细胞较成熟，软骨基质内仍可见材料残留，12周时软骨基质材料残留基本消失。两组组织工程软骨特殊染色与免疫组织化学检测均显示再生软骨胶原与基质黏多糖生成良好，软骨中均检测出Ⅱ型胶原。表明两种材料作为软骨组织工程支架具有良好的适用性，其降解时间均达到组织工程软骨构建的要求。
中国组织工程研究杂志出版内容重点：生物材料；骨生物材料;
口腔生物材料;
纳米材料;
缓释材料;
材料相容性；组织工程
全文链接：
关键词
：
生物材料；
软骨生物材料；
聚羟基乙酸；
聚羟基丁酸己酸共聚物；
适用性；
降解性；
组织工程；
软骨；
体内；
Abstract：
BACKGROUND:
Both
polyglycolic
acid
(PGA)
nonwoven
mesh
and
poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)
(PHBHH)
show
good
applicability,biodegradability
and
biocompatibility
with
porous
sponges.
OBJECTIVE:
To
explore
the
applicability
and
biodegradability
in
vivo
of
PGA
nonwoven
mesh
and
PHBHH
porous
sponge
as
scaffolds
for
tissue
engineered
cartilage.
METHODS:
Rabbit
chondrocytes
were
seeded
onto
PGA
nonwoven
mesh
and
PHBHH
porous
sponge
respectively
as
scaffolds
to
form
chondrocyte-PGA
or
chondrocyte-PHBHH
composites
by
tissue
engineering
technique.
The
composites
were
implanted
subcutaneously
into
the
dorsum
of
the
adult
New
Zealand
white
rabbits
as
experimental
group.
In
addition,PGA
nonwoven
mesh
and
PHBHH
porous
sponge
respectively
were
implanted
subcutaneously
into
the
dorsum
of
another
rabbits
as
control
group.
RESULTS
AND
CONCLUSION:
Histological
examination
showed
that
for
the
tissue
engineered
cartilage
using
PGA
nonwoven
mesh
as
scaffolds
a
few
smaller
chondrocytes
and
some
remainder
of
PGA
fibers
existed
among
the
matrix
of
neocartilage
after
4
weeks;
near
mature
cartilage
appeared
without
PGA
fiber
remainder
after
8
weeks;
then
mature
cartilage
formed
with
a
plenty
of
cartilage
matrix
and
without
PGA
fiber
remainder
after
12
weeks.
For
the
tissue
engineered
cartilage
using
PHBHH
porous
sponge
as
scaffolds,immature
chondrocytes
presented
with
more
remainder
of
PHBHH
similar
to
“foreign
substance”
in
neocartilage
after
4
weeks;
and
some
remainder
of
PHBHH
still
remained
after
8
weeks;
up
until
12
weeks,the
remainder
of
PHBHH
was
invisible
within
the
new
cartilage.
It
was
demonstrated
to
form
rich
cartilaginous
matrix
and
type
II
collage
through
special
staining
and
immunohistochemical
test
at
12
weeks
after
implantation.
Both
PGA
nonwoven
mesh
and
PHBHH
porous
sponge
have
good
applicability
and
biodegradability
as
scaffolds
for
tissue
engineered
cartilage
in
vivo,and
their
biodegradability
meets
the
requirement
of
constructing
tissue
engineered
cartilage.
中国组织工程研究杂志出版内容重点：生物材料；骨生物材料;
口腔生物材料;
纳米材料;
缓释材料;
材料相容性；组织工程
全文链接：
Key
words：
biocompatible
materials;
biodegradation,environmental;
cartilage;
tissue
engineering;
收稿日期:
2013-12-07
中图分类号:
R318
基金资助:
沈阳军区总医院院级重点基金课题(zy2009z0019)
通讯作者:
华泽权，主任医师，解放军沈阳军区总医院颌面外科，辽宁省沈阳市
110016
作者简介:
孙安科，男，1962年生，河南省南阳市人，汉族，1985年解放军第二军医大学毕业，博士后，主任医师，主要从事软骨组织工程与喉软骨功能重建研究。
引用本文:
孙安科,孙
靖,孙
伟等.
无纺网与多孔海绵状材料作为软骨组织工程支架的适用性及体内降解性[J].
中国组织工程研究,2014,18(8):
1172-1178.